18
Oct
2011

 

This week in the labs I have been continuing to test various samples using PCR and gel electrophoresis.  This includes samples from the study I have previously mentioned, where I was testing for competency of the cat flea as a vector for Bartonella Henselae. This week I have also carried out manual DNA extractions on a few pieces of tissue. This process involved lysis of the tissue using protinase K, then cycles of adding various buffers and centrifugation. One of the samples yielded a very high DNA concentration as measured by the nanodrop spectrophotometer, so I had to repeat the last step in the process in order to dilute the DNA concentration as otherwise it would be too high to complete PCR and Gel electrophoresis and the results gained would not have been accurate.

This week I have also received the DNA sequencing results back from the positive samples I had obtained from the Canadian dog blood. Unfortunately, all the sequencing reactions failed to yield sequences that matched what we were looking for. This is a shame as it would have been nice to have been got one positive from all of my samples tested. However, this could be a good thing in the long run as this may mean that Bartonella species, especially Bartonella vinsonni subspecies berkhoffii type 4 has been eliminated from this area. However, more information is required before this assumption can be substantiated, including repeating the DNA sequencing.

Posted in Uncategorized | 72 Comments

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