23
Sep
2011

Monday morning was the second time I’d been down to the necropsy lab. Several post mortems of pigs were underway!! I was able to watch the removal of the dorsal root ganglia. This process took a few hours, but was incredible to watch from an anatomical perspective.  I also watched a cow post mortem. The cow had died from septic mastitis, according to the veterinary pathologist I was assisting.

For the rest of the week I did more Western Blots with the hope of finally obtaining some results by changing yet more variables and altering the concentrations of the primary and secondary concentrations- but, still with no luck!! The results seem to be getting worse, which may suggest that the mucosal scrape samples have degraded over time. I think next week will be our last week of trying to get this technique to work. To try and obtain results we can also perform an ELISA which again uses antibodies to bind to protein. This method can not only detect the presence of a protein, but also quantify it. It is a much more sensitive procedure, but cost three times as much as a Western Blot!!

Posted in Uncategorized | 112 Comments

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